ELEKTRON MİKROSKOPİK ÇALIŞMALARDA İMMUNOGOLD ETİKETLEME TEKNİKLERİ
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immunogold etiketleme, elektron mikroskopi, gömme sonrası etiketleme, gömme öncesi etiketlemeAbstract
Elektron mikroskobik çalışmalarda immün etiketleme teknikleri biyolojik moleküllerin doğal durumlarındaki lokalizasyonlarını olabildiğince koruyarak hata payını en aza indirgeyecek şekilde belirlemeye ve bunu görüntülemeye yarayan teknikleridir. Temeli immun çalışmalardaki antijen antikor ilişkisine dayanılarak geliştirilmiştir. İmmunogold etiketleme, protein çalışmaları ve hücrelerde indikator yapıların varlığını saptamak için de çok güçlü bir tekniktir. Hücrelerin ultrayapısal olarak problanabildiği bir teknik olup kullanılan altın probun mükemmel elektron saçılma özelliği sayesinde elektron mikroskobik immun çalışmalar görüntüleme bakımından da önemli çalışmalardır. Bu derlemede rutin elektron mikroskobik çalışmalarda kullanılabilecek modifikasyonlarla immun etiketleme yöntemlerinden ve immun çalışmalara özel geliştirlmiş tekniklerden bahsedilecektir.
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