Farklı Biyolojik Örnekler Geçirimli Elektron Mikroskop için Nasıl Hazırlanır?

Abstract

Transmission electron microscopes are devices that introduce very important data in the field of cell biology and biomedical researchs. With the
developments new technologies, samples are visualized better. Thus, both our knowledges about biological sample increase and we can reach
the detailed information about the location of important molecules. Although many types of microscopy developed to date, the most advantage
of electron microscopes are the ability to give high resolution and the introduce very detailed information about cell architecture. To visualize
sample in a state as close to a living state as possible, their structural components should be maintained and the determination and application
of available preparation protocole is essential. This review aims to introduce an general point of view about the mostly used techniques about
TEM imaging of biological samples. Both conventional and latest criyo techniques are defined, the advantages and disadvantages of each
technique and introduced knowledges are detailed.